The evaluation of oxidative stress and quality of spermatozoa in Padanian barbel Barbus plebejus and chub Squalius orientalis
DOI:
https://doi.org/10.5281/zenodo.6497971Keywords:
Squalius orientalis, Barbus plebejus, sperm, oxidative stressAbstract
The decrease in quality and death of sperm cells are triggered due to the generation of reactive oxygen species (ROS). Hence, the assessment of oxidative damage and antioxidant status are important. For this purpose, activity of antioxidant enzymes [catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)] and lipid peroxidation [Malondialdehyde (MDA)] were examined in sperm cells of Padanian barbel (Barbus plebejus) and chub (Squalius orientalis). In addition, duration and percentage of spermatozoa were assessed. Based on our data, GPx (43.47±0.02 U g-1 protein) and malondialdehyde (2.52±0.02 nmol.g-1 cell) levels in B. plebejus were higher compared to spermatozoa of S. orientalis. Collectively, alterations in malondialdehyde levels and the antioxidant status could be species-specific differences.
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