Partial replacement of fishmeal with duckweed (Lemna minor) improves growth performance and body composition of Nile tilapia (Oreochromis niloticus) L.
DOI:
https://doi.org/10.5281/zenodo.14555064Abstract
Duckweed (Lemna minor) was evaluated as an alternative protein source to fishmeal for Nile tilapia (Oreochromis niloticus) growth. Nile tilapia juveniles were fed experimental diet with L. minor supplementing fish meal at 0% (LM0), 5% (LM5), and 15% (LM15) and compared to a commercial diet as a positive control (COMM). The diets were administered to O. niloticus juveniles with an initial mean weight of 30.5 g stocked in cages in a pond at 30 fish per cage for 84 days. Growth performance, feed utilization, and body composition were evaluated and compared with those of the control. The final weight and specific growth rate were significantly higher in the fish fed the commercial diet than in LM15 when compared to LM0 (P < 0.05). The fish were able to utilize the L. minor-based feed well, although the feed conversion ratio was significantly lower in fish fed the LM15 and COMM diets (P < 0.05). Fish body composition was significantly affected by L. minor based diets. Protein content was significantly higher in fish fed the control diet and diet LM10 (P < 0.05), whereas lipid content was significantly higher in fish fed duckweed-based diets than in the control, with LM15 having the highest levels (P < 0.05). Partial replacement of fish meal with L. minor at 15% in the diet of O. niloticus is therefore recommended because it enhances growth performance, improves feed utilization, and increases the lipid content in Nile tilapia
References
Abowei, J. F. N., & Ekubo, A. T. (2011). A Review of conventional and unconventional feeds in fish nutrition. British Journal of Pharmacology and Toxicology, 2(4), 179–191.
AOAC. (2023). Official Methods of Analysis of the Association of Official Analytical Chemists (K. Helrich (ed.); 22nd ed.). Association of Official Analytical Chemists (AOAC). https://law.resource.org/pub/us/cfr/ibr/002/aoac.methods.1.1990.pdf
Bairagi, A., Sarkar Ghosh, K., Sen, S., & Ray, A. (2002). Duckweed (Lemna polyrhiza) leaf meal as a source of feedstuff in formulated diets for rohu (Labeo rohita Ham.) fingerlings after fermentation with a fish intestinal bacterium. Bioresource Technology, 85(1), 17–24. https://doi.org/10.1016/S0960-8524(02)00067-6
Caspers, H. (1969). Methods for assessment of fish production in fresh waters. IBP handbook No 3. 3G 8. 6 d. International Review of Hydrobiology, 54(3), 470–471. https://doi.org/10.1002/iroh.19690540313
Chakrabarti, R. (2018). Culture of zooplankton and aquatic macrophytes as non-conventional livelihood. In A. S. Ninawe, J. R. Dhanze, & R. Dhanze (Eds.), Aquaculture for Nutritional and Livelihood Security (pp. 189–203). Narendra Publishing House. https://www.nphindia.com/book/9789386110633/aquaculture-for-nutritional-livelihood-security
Chakrabarti, R., Clark, W. D., Sharma, J. G., Goswami, R. K., Shrivastav, A. K., & Tocher, D. R. (2018). Mass production of Lemna minor and its amino acid and fatty acid profiles. Frontiers in Chemistry, 6, 1–16. https://doi.org/10.3389/fchem.2018.00479
Charo-Karisa, H., Opiyo, M. A., Munguti, J., Marijani, E., & Nzayisenga, L. (2013). Cost-benefit analysis and growth effects of pelleted and unpelleted farm made feeds on african catfish (Clarias gariepinus burchell 1822) in earthen ponds. African Journal of Food, Agriculture, Nutrition and Development, 13(4), 8019–8030. https://www.ajol.info/index.php/ajfand/article/view/94597%0A
Cipriani, L. A., Ha, N., de Oliveira, N. S., & Fabregat, T. E. H. P. (2021). Does ingestion of duckweed (Lemna minor) improve the growth and productive performance of juvenile Nile tilapia (Oreochromis niloticus) given formulated feeds in a recirculation system? Aquaculture International, 29(5), 2197–2205. https://doi.org/10.1007/s10499-021-00743-0
Cruz, Y., Kijora, C., Wedler, E., Danier, J., & Schulz, C. (2011). Fermentation properties and nutritional quality of selected aquatic macrophytes as alternative fish feed in rural areas of the Neotropics. Livestock Research for Rural Development, 23(11), 5–9.
Culley, D. D., Rejmánková, E., Květ, J., & Frye, J. B. (1981). Production, chemical quality and use of duckweeds (Lemnaceae) in aquaculture, waste management, and animal feeds. Journal of the World Mariculture Society, 12(2), 27–49. https://doi.org/10.1111/j.1749-7345.1981.tb00273.x
El-Sayed, A.-F. M. (1999). Alternative dietary protein sources for farmed tilapia, Oreochromis spp. Aquaculture, 179(1–4), 149–168. https://doi.org/10.1016/S0044-8486(99)00159-3
El-Sayed, A. F. M. (2008). Reducing feed costs in semi- intensive tilapia culture. International Aqua Feed, 11(1), 32–34.
El-Shafai, S. A., El-Gohary, F. A., Verreth, J. A. J., Schrama, J. W., & Gijzen, H. J. (2004). Apparent digestibility coefficient of duckweed (Lemna minor), fresh and dry for Nile tilapia (Oreochromis niloticus L.). Aquaculture Research, 35(6), 574–586. https://doi.org/10.1111/j.1365-2109.2004.01055.x
Fasakin, E. A., Balogun, A. M., & Fasuru, B. E. (1999). Use of duckweed, Spirodela polyrrhiza L. Schleiden, as a protein feedstuff in practical diets for tilapia, Oreochromis niloticus L. Aquaculture Research, 30(5), 313–318. https://doi.org/10.1046/j.1365-2109.1999.00318.x
Goswami, R. K., Shrivastav, A. K., Sharma, J. G., Tocher, D. R., & Chakrabarti, R. (2020). Growth and digestive enzyme activities of rohu Labeo rohita fed diets containing macrophytes and almond oil-cake. Animal Feed Science and Technology, 263(January), 114456. https://doi.org/10.1016/j.anifeedsci.2020.114456
Hasan, M. R., & Chakrabarti, R. (2009). Use of algae and aquatic macrophytes as feed in small-scale aquaculture. In FAO Fisheries and Aquaculture Technical Paper 531. Food and Agriculture Organization of the United Nations (FAO). http://www.fao.org/3/a-i1141e.pdf
Hassan, M. S., & Edwards, P. (1992). Evaluation of duckweed (Lemna perpusilla and Spirodela polyrrhiza) as feed for Nile tilapia (Oreochromis niloticus). Aquaculture, 104(3–4), 315–326. https://doi.org/10.1016/0044-8486(92)90213-5
Herawati, V. E., Pinandoyo, P., Darmanto, Y. S., Rismaningsih, N., Windarto, S., & Radjasa, O. K. (2020). The effect of fermented duckweed (Lemna minor) in feed on growth and nutritional quality of tilapia (Oreochromis niloticus). Biodiversitas Journal of Biological Diversity, 21(7), 3350–3358. https://doi.org/10.13057/biodiv/d210759
Kumar, G., Sharma, J., Goswami, R. K., Shrivastav, A. K., Tocher, D. R., Kumar, N., & Chakrabarti, R. (2022). Freshwater macrophytes: A potential source of minerals and fatty acids for fish, poultryand livestock. Frontiers in Nutrition, 9(April), 1–9. https://doi.org/10.3389/fnut.2022.869425
Lambooij, E., Gerritzen, M. A., Reimert, H., Burggraaf, D., & van de Vis, J. W. (2008). A humane protocol for electro-stunning and killing of Nile tilapia in fresh water. Aquaculture, 275(1–4), 88–95. https://doi.org/10.1016/j.aquaculture.2008.01.026
Mbagwu, I. G., Okoye, F. C., & Adeniji, H. A. (1990). Studies on the use of Duckweed (Lemna paucicostata Hegelm.) as fish food. National Institute for Freshwater Fisheries Research. https://aquadocs.org/handle/1834/18705
Montoya-Camacho, N., Marquez-Ríos, E., Castillo-Yáñez, F. J., Cárdenas López, J. L., López-Elías, J. A., Ruíz-Cruz, S., Jiménez-Ruíz, E. I., Rivas-Vega, M. E., & Ocaño-Higuera, V. M. (2019). Advances in the use of alternative protein sources for tilapia feeding. Reviews in Aquaculture, 11(3), 515–526. https://doi.org/10.1111/raq.12243
Mukherjee, A. K., Kalita, P., Unni, B. G., Wann, S. B., Saikia, D., & Mukhopadhyay, P. K. (2010). Fatty acid composition of four potential aquatic weeds and their possible use as fish-feed neutraceuticals. Food Chemistry, 123(4), 1252–1254. https://doi.org/10.1016/j.foodchem.2010.05.057
Naseem, S., Bhat, S. U., Gani, A., & Bhat, F. A. (2021). Perspectives on utilization of macrophytes as feed ingredient for fish in future aquaculture. Reviews in Aquaculture, 13(1), 282–300. https://doi.org/10.1111/raq.12475
Nesan, D., Selvabala, K., & Chan Juinn Chieh, D. (2020). Nutrient uptakes and biochemical composition of Lemna minor in brackish water. Aquaculture Research, 51(9), 3563–3570. https://doi.org/10.1111/are.14693
Noor, J., Hossain, M. A., Bari, M. M., & Azimuddin, K. M. (2000). Effects of duckweed (Lemna minor) as dietary fishmeal substitute for silver barb (Barbodes gonionotus Bleeker). Bangladesh Journal of Fisheries Research, 4(1), 35–42.
Opiyo, M. A., Mbogo, K., Obiero, K., Orina, P., & Muendo, P. (2023). Nutritional composition of duckweed (Lemna minor) cultured with inorganic fertilizer and organic manure in earthen ponds. Journal of Limnology and Freshwater Fisheries Research, 9(3), 123–128. https://doi.org/10.17216/limnofish.1152512
Opiyo, M. A., Muendo, P., Mbogo, K., Ngugi, C. C., Charo-Karisa, H., Orina, P., Leschen, W., Glencross, B. D., & Tocher, D. R. (2022). Inclusion of duckweed (Lemna minor) in the diet improves flesh omega-3 long-chain polyunsaturated fatty acid profiles but not the growth of farmed Nile tilapia (Oreochromis niloticus). Animal Feed Science and Technology, 292(September), 115442. https://doi.org/10.1016/j.anifeedsci.2022.115442
Shafi, J., Waheed, K. N., Mirza, Z. S., & Razaq, S. (2024). Effect of duckweed-based aquafeeds on feed digestibility and growth performance of Gibelion catla. Pakistan Journal of Zoology, 56(3), 1109–1115. https://doi.org/10.17582/journal.pjz/20220722090715
Shati, S. M., Opiyo, M. A., Nairuti, R. N., Shoko, A. P., Munyi, F., & Ogello, E. O. (2022). Black soldier fly (Hermetia illucens) larvae meal improves growth performance, feed utilization, amino acids profile, and economic benefits of Nile tilapia (Oreochromis niloticus, L.). Aquatic Research, 5(3), 238–249. https://doi.org/10.3153/AR22023
Slembrouck, J., Samsudin, R., Pantjara, B., Sihabuddin, A., Legendre, M., & Caruso, D. (2018). Choosing floating macrophytes for ecological intensification of small-scale fish farming in tropical areas: a methodological approach. Aquatic Living Resources, 31, 30. https://doi.org/10.1051/alr/2018017
Solomon, S. G., & Okomoda, V. T. (2012). Growth performance of Oreochromis niloticus fed duckweed (Lemna minor) based diets in outdoor hapas. International Journal of Research in Fisheries and Aquaculture, 2(4), 61–65.
Sosa, D., Alves, F. M., Prieto, M. A., Pedrosa, M. C., Heleno, S. A., Barros, L., Feliciano, M., & Carocho, M. (2024). Lemna minor: Unlocking the value of this duckweed for the food and feed Industry. Foods, 13(10), 1435. https://doi.org/10.3390/foods13101435
Stadtlander, T., Tschudi, F., Seitz, A., Sigrist, M., Refardt, D., & Leiber, F. (2023). Partial replacement of fishmeal with duckweed (Spirodela polyrhiza) in feed for two carnivorous fish species, Eurasian perch (Perca fluviatilis) and rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 2023, 1–15. https://doi.org/10.1155/2023/6680943
Tavares, F. D. A., Rodrigues, J. B. R., Fracalossi, D. M., Esquivel, J., & Roubach, R. (2008). Dried duckweed and commercial feed promote adequate growth performance of tilapia fingerlings. Biotemas, 21(3), 91–97. https://doi.org/10.5007/2175-7925.2008v21n3p91
Uddin, M., Rahman, M., & Shahjahan, M. (2014). Effects of duckweed (Lemna minor) as supplementary feed on monoculture of gift strain of tilapia (Oreochromis niloticus). Progressive Agriculture, 18(2), 183–188. https://doi.org/10.3329/pa.v18i2.18201
Yen, D. T., Thi, T., Hien, T., & Phuong, N. T. (2015). The evaluation of some plants as dietary protein sources for fingerlings of tilapia (Oreochromis niloticus) and silver barb (Puntius gonionotus). Institute for Marine Aquaculture, College of Agriculture, November 2014, 1–7.
Yılmaz, E., Akyurt, I., & Günal, G. (2004). Use of duckweed, Lemna minor, as a protein feed stuff in practical diets for Common carp, Cyprinus carpio, fry. Turkish Journal of Fisheries and Aquatic Sciences, 4(2), 105–109.
Young, D. L. W., Wiegand, M. D., Loadman, N. L., Collins, S. A., Ballevona A, A. J., & Huebner, J. D. (2006). Effects of artificial ultraviolet-B radiation on growth and fatty acid composition of duckweed (Lemna minor). Freshwater Biology, 51(11), 2029–2040. https://doi.org/10.1111/j.1365-2427.2006.01633.x
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