Temperature influences masculinity, sex reversal after mono-sex, and the hormonal residue in the flesh of Nile tilapia (Oreochromis niloticus)


  • Tushar Baadruzzoha Sarker Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet, Bangladesh
  • Mohammad Amzad Hossain Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet, Bangladesh
  • M. M. Mahbub Alam Department of Fish Health Management, Sylhet Agricultural University, Sylhet, Bangladesh
  • Sohel Mian Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet, Bangladesh
  • Mohammed Mahbub Iqbal Department of Fish Biology and Genetics, Sylhet Agricultural University, Sylhet, Bangladesh




sex reversal, mono sex tilapia, masculinization, hormone residue, fish flesh, tilapia aquaculture


This study was conducted to investigate the impact of temperature on masculinity and growth in hatchery and production phases, and to assess the hormonal residue in fish flesh of Nile tilapia (Oreochromis niloticus). In cemented tanks with hapa setting hatchlings were released and fed with 60 g/kg hormone and maintained at 30 ºC, 33 ºC, 36 ºC and 39 ºC for 28 days and 60 days. At 36 ºC no female fish was observed during the 28 days of hormone treatment and 60 days of trial production. The average body weight gain, daily weight gain, growth trends and survival rate were higher in 36 ºC. The 36 °C treatment was repeated in the production pond for 178 days. Several females (5-6 individuals) were discovered. The survival rate, other growth parameters, and water quality parameters were found to be comparatively optimum during the experiment. Another setup was used to assess the hormonal residue and found at the end of the experiment 42.1 pg/mL 17α-methyltestosterone rest in fish muscle was observed in 178 days. The water quality parameters used in this investigation were also consistent and did not change noticeably during the course of the experiment. This study determined the ideal temperature producing mono-sex tilapia in hatchery and production conditions. The results of this study might enable Nile tilapia mono-sex hatcheries to operate more cheaply and produce pure male tilapia, however, it may also help the government establish a limit for the permissible level of hormone residue in commercial tilapia products.


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How to Cite

Baadruzzoha Sarker, T., Mohammad Amzad Hossain, M. M. Mahbub Alam, Sohel Mian, & Mohammed Mahbub Iqbal. (2023). Temperature influences masculinity, sex reversal after mono-sex, and the hormonal residue in the flesh of Nile tilapia (Oreochromis niloticus). Sustainable Aquatic Research, 2(1), 1–23. https://doi.org/10.5281/zenodo.7881895



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